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BS EN 14122:2014 Foodstuffs. Determination of vitamin B1 by high performance liquid chromatography >
BS EN 14122:2014 Foodstuffs. Determination of vitamin B1 by high performance liquid chromatography, 2014
- Foreword
- 1 Scope
- 2 Normative references
- 3 Principle
- 4 Reagents
- 5 Apparatus
- 6 Procedure [Go to Page]
- 7 Calculation
- 8 Precision [Go to Page]
- 9 Test report
- Annex A (informative) Examples of HPLC chromatograms
- Figure A.1 — Example for an HPLC separation of thiamin as thiochrome standard using pre-column derivatization
- Figure A.2 — Examples for a HPLC separation of thiamin using post-column derivatization in lettuce (a), cooked rice (b) and cooked pork meat (c)
- Annex B (informative) Precision data
- Table B.1 — Precision data for whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver
- Table B.2 — Precision data for tube feeding solution, baby food with vegetables, powdered milk, meal with fruits and yeast
- Table B.3 — Precision data for cereal, chocolate powder and food supplement
- Annex C (informative) Alternative HPLC systems
- Table C.1 — Alternative HPLC conditions
- Annex D (informative) Vitamin B1 compound: 2-(1-hydroxyethyl)thiamin (HET) performing post-column derivatization
- Figure D.1 — Chromatogram of an extract of CRM 487 pigs liver (a) and a standard of 0,1 µg/ml TMP (Thiamin Mono Phosphate), 0,1 µg/ml thiamin and 0,01 µg/ml HET (2-(1-hydroxyethyl)thiamin) (b)
- Bibliography [Go to Page]