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BS EN 17914:2025 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of virucidal activity in the food, industrial, domestic and institutional area. Test method and requirements (Phase 2/Step 1) >
BS EN 17914:2025 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of virucidal activity in the food, industrial, domestic and institutional area. Test method and requirements (Phase 2/Step 1), 2025
- undefined
- European foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Requirements
- Table 1 — Minimum and additional test conditions for professional users, non-medical
- Table 2 — Minimum and additional test conditions for non-professional users, non-medical
- 5 Test methods [Go to Page]
- Table 3 — Consolidated classification of water qualities [Go to Page]
- Table 4 — Required chemical and physical parameters [Go to Page]
- Table 5 — Limits for reference substances [Go to Page]
- Annex A (informative) Viruses covered by virucidal activity claims described in this document
- Annex B (informative) Detoxification of test mixtures
- B.1 Molecular sieving with SephadexTM LH 204F
- B.1.1 Principle
- B.1.2 Sephadex suspension
- B.1.3 Procedure
- Figure B.1 — Molecular sieving device with syringe
- B.2 Molecular sieving using MicroSpin™ S 400 HR
- Figure B.2 — MicroSpin™ S 400 HR for purification of virus samples
- B.3 Determination of the residual virus titre by the large-volume-plating (LVP) method
- B.3.1 General
- B.3.2 Example for the calculation of titres and the reduction according to the LVP method
- Annex C (informative) Calculation of the viral infectivity titre
- C.1 Quantal tests — Example of TCID50 determination by the Spearman-Kärber method
- Table C.1 — Exemplary values for a test result with 6 wells
- Table C.2 — Exemplary values for a test result
- C.2 Plaque test
- C.3 Biometrical evaluation of experimental approaches and assessment of the disinfecting effect on the virus (reduction [R])
- C.3.1 General
- C.3.2 Calculating the virus titre
- C.3.3 Calculating the reduction and its 95 % confidence interval
- Annex D (informative) Presentation of test results of one active concentration
- Table D.1 — Table of results of product XYZ and vaccinia virus under dirty conditions
- Table D.2 — Raw data for product XYZ with 3 g/l BSA tested against vaccinia virus (quantal test; e.g. 8 wells)
- Annex E (informative) Preparation of the glutaraldehyde test solutions (v/v)
- Table E.1 — The dilutions of the reference product glutaraldehyde
- Bibliography [Go to Page]