eLibrary >
BS EN 17915:2025 Chemical disinfectants and antiseptics. Quantitative non-porous surface test without mechanical action for the evaluation of virucidal activity of chemical disinfectants used in the food, industrial, domestic and institutional area. Test >
BS EN 17915:2025 Chemical disinfectants and antiseptics. Quantitative non-porous surface test without mechanical action for the evaluation of virucidal activity of chemical disinfectants used in the food, industrial, domestic and institutional area. Test , 2025
- undefined
- European foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Requirements for virucidal activity on surfaces
- Table 1 — Minimum and additional test conditions for professional users, non-medical
- Table 2 — Minimum and additional test conditions for non-professional users, non-medical
- 5 Test methods [Go to Page]
- Table 3 — Consolidated classification of water qualities [Go to Page]
- Table 4 — Required chemical and physical parameters [Go to Page]
- Table 5 — Limits for reference substances [Go to Page]
- Annex A (informative) Detoxification of test mixtures by molecular sieving
- A.1 Molecular sieving with Sephadex™ LH 206F
- A.1.1 Principle
- A.1.2 Sephadex suspension
- A.1.3 Procedure
- Figure A.1 — Molecular sieving device with syringe
- A.2 Molecular sieving using MicroSpin™ S 400 HR
- Figure A.2 — MicroSpin™ S 400 HR for purification of virus samples
- A.3 Determination of the residual virus titre by the large-volume-plating (LVP) method
- A.3.1 General
- A.3.2 Example for the calculation of titres and the reduction according to the LVP method
- Annex B (informative) Calculation of the viral infectivity titre
- B.1 Quantal tests — Example of TCID50 determination by the Spearman-Kärber method
- Table B.1 — Example values for a test results with 6 wells
- Table B.2 — Exemplary values for a test result
- B.2 Plaque test
- B.3 Biometrical evaluation of experimental approaches and assessment of the disinfecting effect on the virus (reduction [R])
- B.3.1 General
- B.3.2 Calculating the virus titre
- B.3.3 Calculating the reduction and its 95 % confidence interval
- B.3.4 Practical example
- B.3.4.1 Introduction
- Table B.3 — Raw data for the example of Titration results – Before (virus control titration = a)
- Table B.4 — Raw data for the example of Titration results – After exposure to product (rest virus = b)
- B.3.4.2 Calculating the reduction and its 95 % confidence interval:
- B.3.4.3 Calculating the reduction and its 95 % confidence interval
- B.3.4.4 Calculating the average reduction [R(mi)] and its 95 % confidence interval
- Annex C (informative) Presentation of test results of one active concentration
- Table C.1 — Table of results of product XYZ and vaccinia virus under dirty conditions at room temperature
- Table C.2 — Raw data for product XYZ with 3 g/l BSA tested against vaccinia virus
- Annex D (informative) Preparation of the glutaraldehyde test solutions (v/v)
- Table D.1 — The dilutions of the reference product glutaraldehyde
- Bibliography [Go to Page]