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eLibrary > PD CEN ISO/TS 15216-2:2013 Microbiology of food and animal feed. Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Method for qualitative detection >

PD CEN ISO/TS 15216-2:2013 Microbiology of food and animal feed. Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Method for qualitative detection, 2013

PD CEN ISO/TS 15216-2:2013 Microbiology of food and animal feed. Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Method for qualitative detection, 2013

Section sec_1
Section sec_2
Section sec_3
Section sec_3.1
Section sec_3.2
Section sec_3.3
Section sec_3.4
Section sec_3.5
Section sec_3.6
Section sec_3.7
Section sec_3.8
Section sec_3.9
Section sec_3.10
Section sec_3.11
Section sec_3.12
Section sec_3.13
Section sec_3.14
Section sec_3.15
Section sec_3.16
Section sec_3.17
Section sec_4
Section sec_4.1
Section sec_4.2
Section sec_4.3
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.5
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.2.1
Section sec_5.2.2
Section sec_5.2.3
Section sec_5.2.4
Section sec_5.2.5
Section sec_5.2.6
Section sec_5.2.7
Section sec_5.2.8
Section sec_5.2.9
Section sec_5.2.10
Section sec_5.2.11
Section sec_5.2.12
Section sec_5.2.13
Section sec_5.2.14
Section sec_5.2.15
Section sec_5.2.16
Section sec_5.2.17
Section sec_5.2.18
Section sec_5.2.19
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.3.4
Section sec_5.3.5
Section sec_5.3.6
Section sec_5.3.7
Section sec_5.3.8
Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.3
Section sec_6.4
Section sec_6.5
Section sec_6.6
Section sec_6.7
Section sec_6.8
Section sec_6.9
Section sec_6.10
Section sec_6.11
Section sec_6.12
Section sec_6.13
Section sec_6.14
Section sec_6.15
Section sec_6.16
Section sec_6.17
Section sec_6.18
Section sec_6.19
Section sec_6.20
Section sec_6.21
Section sec_6.22
Section sec_6.23
Section sec_6.24
Section sec_6.25
Section sec_6.26
Section sec_6.27
Section sec_6.28
Section sec_7
Section sec_8
Section sec_8.1
Section sec_8.2
Section sec_8.2.1
Section sec_8.2.2
Section sec_8.2.3
Section sec_8.2.4
Section sec_8.2.5
Section sec_8.2.6
Section sec_8.3
Section sec_8.4
Section sec_8.4.1
Section sec_8.4.2
Section sec_8.4.2.1
Section sec_8.4.2.2
Section sec_8.4.2.3
Section sec_8.4.2.4
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.3
Section sec_9.4
Section sec_9.5
Section sec_10
Section sec_11
Annex sec_A
Annex sec_B
Table tab_B.1
Table tab_B.2
Annex sec_C
Annex sec_C.1
Annex sec_C.2
Annex sec_C.3
Annex sec_C.4
Annex sec_D
Annex sec_D.1
Annex sec_D.2
Annex sec_D.2.1
Annex sec_D.2.2
Annex sec_D.3
Annex sec_E
Annex sec_E.1
Annex sec_E.1.1
Annex sec_E.2
Annex sec_E.2.1
Annex sec_E.2.2
Annex sec_E.2.3
Annex sec_E.2.4
Annex sec_E.3
Annex sec_F
Annex sec_F.1
Annex sec_F.1.1
Annex sec_F.1.2
Annex sec_F.2
Annex sec_F.2.1
Annex sec_F.2.2
Annex sec_F.3
Annex sec_F.3.1
Annex sec_F.3.2
Annex sec_F.4
Annex sec_F.4.1
Annex sec_F.4.2
Annex sec_F.5
Annex sec_F.5.1
Annex sec_F.5.2
Annex sec_G
Annex sec_G.1
Annex sec_G.2
Annex sec_G.2.1
Annex sec_G.2.2
Annex sec_G.2.3
Annex sec_G.2.4
Annex sec_G.2.5
Annex sec_G.2.6
Annex sec_G.2.7
Annex sec_G.2.8
Annex sec_G.3
Annex sec_G.4
Annex sec_G.5
Annex sec_G.6
Annex sec_H
Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Reference ref_6
Reference ref_7
Reference ref_8
Reference ref_9
Reference ref_10
Reference ref_11
Reference ref_12
Annex E (informative) RNA extraction using the BioMerieux NucliSens® system
Annex F (normative) Composition and preparation of reagents and buffers
Annex G (informative) Generation of external control RNA (EC RNA) stocks
Annex H (informative) Typical optical plate layout
Bibliography
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Virus extraction
4.2 RNA extraction
4.3 Real-time reverse transcription polymerase chain reaction (real-time RT-PCR)
4.4 Control materials
4.5 Test results
5 Reagents
5.1 General
5.2 Reagents used as supplied
5.3 Prepared reagents
6 Apparatus and materials
7 Sampling
8 Procedure
8.1 General laboratory requirements
8.2 Virus extraction
8.3 RNA extraction
8.4 Real-time RT-PCR
9 Interpretation of results
9.1 General
9.2 Construction of process control virus RNA standard curve
9.3 Control for amplification efficiency
9.4 Calculation of extraction efficiency
9.5 Theoretical limit of detection
10 Expression of results
11 Test report
Annex A (normative) Diagram of procedure
Annex B (informative) Real-time RT-PCR mastermixes and cycling parameters
Annex C (informative) Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control)
Annex D (informative) Growth of mengo virus strain MC0 for use as a process control
Annex E (informative) RNA extraction using the BioMerieux NucliSens® system
Annex F (normative) Composition and preparation of reagents and buffers
Annex G (informative) Generation of external control RNA (EC RNA) stocks
Annex H (informative) Typical optical plate layout
Bibliography [Go to Page]

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PD CEN ISO/TS 15216-2:2013 Microbiology of food and animal feed. Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Method for qualitative detection, 2013

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