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PD CEN/TS 16621:2014 Food analysis. Determination of benzo[a]pyrene, benz[a]anthracene, chrysene and benzo[b]fluoranthene in foodstuffs by high performance liquid chromatography with fluorescence detection (HPLC-FD) >
PD CEN/TS 16621:2014 Food analysis. Determination of benzo[a]pyrene, benz[a]anthracene, chrysene and benzo[b]fluoranthene in foodstuffs by high performance liquid chromatography with fluorescence detection (HPLC-FD), 2014
- Foreword
- 1 Scope
- 2 Normative references
- 3 Principle
- 4 Reagents
- 5 Apparatus
- 6 Procedure [Go to Page]
- 7 HPLC analysis [Go to Page]
- Table 1 — Gradient conditions
- Table 2 — Fluorescent wavelength programme [Go to Page]
- Table 3 — Preparation of HPLC calibration solutions [Go to Page]
- 8 Calculation
- 9 Recovery
- 10 Test report
- 11 Precision data [Go to Page]
- Annex A (informative) Typical chromatograms
- Figure A.1 — Typical chromatogram of 15+1 PAHs from a sample of fresh mussels spiked at a level of 10 μg/kg, each (CPP cannot be detected by fluorescence at that level)
- Figure A.2 — Typical chromatogram of 15+1 PAHs from a sample of extra virgin olive oil spiked at a level of 2 μg/kg, each (CPP is cannot be detected by fluorescence at that level)
- Figure A.3 — Typical chromatogram of the PAH4 from a reference material of olive oil containing 2,06 μg/kg of BaP, 3,17 μg/kg of BaA, 2,40 μg/kg of BbF and 9,52 μg/kg of CHR, under the specific chromatographic conditions for the determination of the PAH4
- Figure A.4 — Typical chromatogram from a sample of processed cereal-based foods for young children spiked with the 15+1 PAH at the level of 10 μg/kg each, under the specific chromatographic conditions for the determination of the 15+1 PAHs. The chro...
- Annex B (informative) In-house validation data for the PAH4 in different matrices
- Table B.1 — In-house validation data for edible olive oil
- Table B.2 — In-house validation data for fresh mussels
- Table B.3 — In-house validation data for smoked fish
- Table B.4 — In-house validation data for smoked meat products
- Table B.5 — In-house validation data for processed cereal-based foods for young children
- Table B.6 — In-house validation data for infant formulae
- Table B.7 — In-house validation data for chocolate
- Table B.8 — In-house validation data for food supplements (isoflavones)
- Annex C (informative) In-house performance data with a mixture of cyclohexane and ethyl acetate as alternative extraction solvent
- Table C.1 — In-house performance data for olive oil
- Table C.2 — In-house performance data for fresh mussel
- Table C.3 — In-house performance data for smoked fish
- Table C.4 — In-house performance data for infant formulae
- Bibliography [Go to Page]