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ISO/TS 18827:2017 Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials >
ISO/TS 18827:2017 Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials, 2017
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms, definitions and abbreviations
- 3.1 Terms and definitions
- 3.2 Abbreviations
- 4 Principle
- 4.1 General
- 4.2 Spin trapping method
- 4.2.1 General
- 4.2.2 DMPO
- 4.2.3 BMPO
- 4.2.4 TPC
- 4.3 Positive control for generating free radicals
- 4.3.1 Fenton reaction[14]
- 4.3.2 Hypoxanthine–xanthine oxidase system[15]
- 4.3.3 Rose bengal photosensitization[16][17]
- 5 Reagents
- 6 Apparatus
- 7 Sampling
- 7.1 Preparation of test sample (metal oxide nanomaterial suspension)
- 7.2 Preparation of solution for generating the hydroxyl radical
- 7.2.1 FeSO4 solution
- 7.2.2 H2O2 solution
- 7.3 Preparation of solution for generating the superoxide anion radical
- 7.3.1 Phosphate buffer
- 7.3.2 Hypoxanthine solution
- 7.3.3 Xanthine oxidase solution
- 7.4 Preparation of solution for generating the singlet oxygen
- 7.5 Preparation of spin trapping agent
- 7.5.1 General
- 7.5.2 DMPO stock solution
- 7.5.3 BMPO stock solution
- 7.5.4 TPC stock solution
- 7.6 Reaction of test sample and spin trapping agent
- 7.6.1 General
- 7.6.2 DMPO reaction
- 7.6.3 BMPO reaction
- 7.6.4 TPC reaction
- 7.7 Reaction of positive control and spin trapping agent
- 7.7.1 DMPO radical adduct form (DMPO/OH)
- 7.7.2 BMPO radical adduct form (BMPO/OOH)
- 7.7.3 TPC radical adduct form (TPC/1O2)
- 7.8 Preparation of the standard sample for spin calculation
- 8 Interferences
- 8.1 Sampling
- 8.2 Sampling time
- 9 Procedure
- 9.1 General
- 9.2 Injection of sample
- 9.3 ESR measurement
- 10 Examples of results
- 10.1 DMPO/OH
- 10.2 BMPO/OOH
- 10.3 TPC/1O2
- 10.4 TEMPOL
- Bibliography [Go to Page]