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Description of ASTM-D8527 2025ASTM D8527-25Active Standard: Standard Test Method for Assessment of Fungal Biomass on Surfaces or Coatings by Measuring Fungal β-N-acetylhexosaminidase (NAHA) ActivityASTM D8527Scope 1.1 This test method contains procedures for both collection and analysis of surface swab samples for total fungal biomass by measuring the activity of fungal β-N-acetylhexosaminidase (NAHA) by use of a 4-methylumbelliferyl fluorogenic enzyme substrate together with an inhibitor of non-fungal NAHA activity. 1.2 NAHA activity is present in all forms of fungal material such as spores, hyphal fragments, and other particles of fungal origin that are not optically recognizable. Collectively, this material is the fungal biomass. See Refs (1-4).2 1.3 This test method can quantify surface fungal biomass loading for comparison purposes (1-4). 1.4 This test method does not identify or differentiate fungal taxa. 1.5 This test method does not differentiate between viable and non-viable fungi. 1.6 This test method can be used to supplement visual observations during fungal assessments and fungal post-remediation verification (PRV). 1.7 This test method does not measure bacteria. 1.8 This test method may involve potentially hazardous chemicals and microorganisms, and should be performed only by persons who have had appropriate training. 1.9 Units—The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard. For purposes of this standard, these terms have no technical meaning. 1.10 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.11 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. Keywords β-N-acetylhexosaminidase; building materials; enzyme activity; fluorescent count; fluorescent unit; fluorimetry; fluorometer; fungal biomass; fungal growth; microbiological assay; mold; NAHA; ICS Code ICS Number Code 07.100.99 (Other standards related to microbiology) DOI: 10.1520/D8527-25 This book also exists in the following packages...Subscription InformationMADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
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About ASTMASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide. |
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