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Description of ASTM-E3527 2026ASTM E3527-26Active Standard: Standard Guide for Preservation of MicroorganismsASTM E3527Scope 1.1 There is an ongoing need to maintain, preserve, and standardize microbiological cultures for research. This guide is focused on three parts of microbe preservation: 1.1.1 Process Description—This includes detailed microbe characterization data and documentation of microbial passaging, measurable specifications (including titers and solution concentrations), and storage conditions (for example, wet or dry storage and temperatures). Characterization data maintained from the origin of microbial isolation through preservation and disposal is a best practice. Although maintaining the purity of a microbiological culture is ideal in most circumstances, many original environmental isolates or clinical samples may be mixtures. Also, certain organisms, including mutualistic biofilm organisms and lichens or obligate pathogenic organisms (for example, viruses), may be best preserved in conjunction with symbiotic organisms or host cells. 1.1.2 Microbe Preservation Tools—Descriptions, monitoring, and maintenance of equipment used in preservation may be documented in laboratory plans or standard operating procedures (SOPs), or preferably both as a best practice. Also, detailed documentation of any personal protective equipment (PPE) requirements and the various mechanisms for administrative control (for example, controlled access to organisms) and engineering control (maintenance of sterile culture and verifications of identities of preserved microbes) may be maintained. 1.2 In this guide, methods for characterization, storage, maintenance, and stabilization of a broad taxonomy of microorganisms are reviewed, including vegetative bacteria, bacterial spores, mycobacteria, fungi, and viruses. 1.3 This guide is intended to assist in the development of standardized methods for preservation of microorganisms by indicating actions to be considered at each stage of accession and preservation. 1.4 This guide does not purport to detail the specific workflow necessary to developing a standardized method for preservation of a specific microorganism. Literature review and empirical evidence may be considered in the process of developing a final method specific to the microorganism of interest. 1.5 Units—The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. Keywords ambient; bacteria; cryogenic; freeze-drying; freezing; fungi; microorganisms; preservation; storage; virus; ICS Code ICS Number Code n/a DOI: 10.1520/E3527-26 This book also exists in the following packages...Subscription InformationMADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
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About ASTMASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide. |
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